ｃ；Plating the seeds on agar medium |
| After burning the opening of the flask with rubber cap, take off the cap with aluminum] foil. You had better keep the rubber cap somewhere is higher than your handling. When you were skillful for the procedure, you will be able to keep the cap on the right hand between the little finger and the palm while you were plating. |
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（８）By your left hand's tweezers keeping the pod like in the fig, you rake the seeds from pod by a sterile spoon on the agar in the flasks. After burning the opening of flask again and close the rubber cap. You had better plating another half of the seeds of pod will be plated in the another flask. |
| （９）When you finished all seeds to be plated,
you put about 0.5 ~ 1.0 ml of sterile distilled water on the
seeds in flasks. （１０）After sterilized by burning the opening of flask, close the rubber cap tightly. And then shake the flask softly to disperse the seeds on agar and to become familiar with the seeds. If the agar would be so soft, you don't need to put water any more. In this case, you disperse the seeds on agar with sterile spoon or stick. |
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